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dio tre hm4di p2a m cherry  (Addgene inc)


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    Structured Review

    Addgene inc dio tre hm4di p2a m cherry
    KEY RESOURCES TABLE
    Dio Tre Hm4di P2a M Cherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dio+tre+hm4di+p2a+m+cherry/pAAV-RAM-d2TTA%3A%3ATRE-MCS-WPRE-pA+(Plasmid+%2363931)/pmc11080946-244-26-38
    Average 93 stars, based on 18 article reviews
    dio tre hm4di p2a m cherry - by Bioz Stars, 2026-11
    93/100 stars

    Images

    1) Product Images from "Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli"

    Article Title: Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli

    Journal: Cell reports

    doi: 10.1016/j.celrep.2024.114009

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Plasmid Preparation, Recombinant, Software

    Related Articles

    Injection:

    Article Title: Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli
    Article Snippet: Commercially available peanut-butter oil was used as a distinct APP odor, and peppermint oil (Sigma # 77411) was used as a distinct AV odor. .. A Hamilton syringe was slowly lowered to the VP and mice were injected with ~0.3 μL of the following viral vectors dependent on experiment: AAV 9 .DIO.TRE.hM4Di.P2A.m.Cherry (ADCD; packaged at UNC viral vector core), AAV 9 .RAM.d2tTA.TRE.mCherry.NLS-FLAG (RAM; Addgene # 63931, packaged at UNC viral vector core), AAV 9 .Syn.Flex-GCaMP6f.WPRE.SV40 (GCaMP6f; Addgene # 100837), AAV 8 .hSyn.DIO.hM4Di (Gi).mCherry (hM4Di; Addgene # 44362), AAV 9 .eSyn.eGFP (Vector Biolabs # VB4870), AAV 9 .RAM.d2tTA.TRE.Flex.tdTomato (FLEX-RAM; Addgene # 84468, packaged at NINDS Viral Vector Core). .. For retrograde tracing experiments, 0.15 μL Fast Blue (Polysciences # 17740-1) was injected bilaterally in the BLA (AP −1.35, ML +/− 3.2, DV 4.7).

    Bioprocessing:

    Article Title: Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli
    Article Snippet: Commercially available peanut-butter oil was used as a distinct APP odor, and peppermint oil (Sigma # 77411) was used as a distinct AV odor. .. A Hamilton syringe was slowly lowered to the VP and mice were injected with ~0.3 μL of the following viral vectors dependent on experiment: AAV 9 .DIO.TRE.hM4Di.P2A.m.Cherry (ADCD; packaged at UNC viral vector core), AAV 9 .RAM.d2tTA.TRE.mCherry.NLS-FLAG (RAM; Addgene # 63931, packaged at UNC viral vector core), AAV 9 .Syn.Flex-GCaMP6f.WPRE.SV40 (GCaMP6f; Addgene # 100837), AAV 8 .hSyn.DIO.hM4Di (Gi).mCherry (hM4Di; Addgene # 44362), AAV 9 .eSyn.eGFP (Vector Biolabs # VB4870), AAV 9 .RAM.d2tTA.TRE.Flex.tdTomato (FLEX-RAM; Addgene # 84468, packaged at NINDS Viral Vector Core). .. For retrograde tracing experiments, 0.15 μL Fast Blue (Polysciences # 17740-1) was injected bilaterally in the BLA (AP −1.35, ML +/− 3.2, DV 4.7).



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli

    doi: 10.1016/j.celrep.2024.114009

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: A Hamilton syringe was slowly lowered to the VP and mice were injected with ~0.3 μL of the following viral vectors dependent on experiment: AAV 9 .DIO.TRE.hM4Di.P2A.m.Cherry (ADCD; packaged at UNC viral vector core), AAV 9 .RAM.d2tTA.TRE.mCherry.NLS-FLAG (RAM; Addgene # 63931, packaged at UNC viral vector core), AAV 9 .Syn.Flex-GCaMP6f.WPRE.SV40 (GCaMP6f; Addgene # 100837), AAV 8 .hSyn.DIO.hM4Di (Gi).mCherry (hM4Di; Addgene # 44362), AAV 9 .eSyn.eGFP (Vector Biolabs # VB4870), AAV 9 .RAM.d2tTA.TRE.Flex.tdTomato (FLEX-RAM; Addgene # 84468, packaged at NINDS Viral Vector Core).

    Techniques: Virus, Plasmid Preparation, Recombinant, Software